Lipase Production From Free Cells and Immobilized Whole Cells: A Comparative Approach
Saranya.N, Padma Gangeshwari. A
Lipases play a vital role in modern Biotechnology owing to their multifaceted properties. Their production and purification procedures need to be improved in order to enhance their stability and turn over. This work was done to study the effect of immobilization of whole cells of lactobacillus in the production of lipase in comparison with free cells of lactobacillus. Ammonium sulphate precipitation and High performance liquid chromatography were used as purification procedures. Activity of purified lipases were found to be 10µmol fatty acid released/ml of substrate for free cell fermentation and 40 µmol fatty acid released/ml of substrate for immobilized cells respectively. Tolerence of lipase against organic solvents such as ethanol, acetone, benzene, and hexane were determined. Michaelis Menton parameters for the enzyme like Vmax, Km ,β were found to be 1.295 µg/ml/min,0.924 µg/ml,1.0663 for free cell fermentation and 0.201µg/ml/min ,9.378µg/ml,10.825 for immobilized whole cell fermentation.
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